Strategies for the Production of Recombinant Protein in Escherichia coli (1) A protein’s intrinsic ability to fold, its solubility, preferential codon use, its toxicity, and…
Science
Protein expression and purification
Cloning technologies for protein expression and purification(1) Protein overexpression requires three components: a gene, a vector that contains the gene, and an expression host that…
BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity
BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity (1) IgAs bind to microbial components…
Airway epithelial cell necroptosis contributes to asthma exacerbation in a mouse model of house dust mite-induced allergic inflammation
Airway epithelial cell necroptosis contributes to asthma exacerbation in a mouse model of house dust mite-induced allergic inflammation (1) Recent research has highlighted the importance…
IgA mesangial deposits in C3H/HeJ mice after oral immunization with ferritin or bovine serum albumin
IgA mesangial deposits in C3H/HeJ mice after oral immunization with ferritin or bovine serum albumin(1) The IgA deposited in the kidney are polymeric and presumably…
EXPERIMENTAL IgA NEPHROPATHY INDUCED BY ORAL IMMUNIZATION*
EXPERIMENTAL IgA NEPHROPATHY INDUCED BY ORAL IMMUNIZATION* (1) An expansion of specific IgA synthesizing cells in mesenteric lymph nodes,spleen,and secretory mucosae and a significant elevation…
Experimental Immunoglobulin A Nephropathy Induced by Gram-Negative Bacteria
Experimental Immunoglobulin A Nephropathy Induced by Gram-Negative Bacteria (1) On the basis of clinical and experimental observations, a number of etiologic candidates for IgA nephropathy…
Mice Refractory to Lipopolysaccharide Manifest High Immunoglobulin A Responses to Orally Administered Antigen
Mice Refractory to Lipopolysaccharide Manifest High Immunoglobulin A Responses to Orally Administered Antigen (1) The interaction of antigen with gut-associated lymphoid tissues is a major…
Epitope characterization of ovalbumin in BALB/c mice using different entry routes
Epitope characterization of ovalbumin in BALB/c mice using different entry routes (1) Ovalbumin is a 385 amino acid glycoprotein of approximately 45 kDa, which constitutes…
Salt-Induced Disaggregation/Solubilization of Gliadin and Glutenin Proteins in Water
Salt-Induced Disaggregation/Solubilization of Gliadin and Glutenin Proteins in Water (1) The limited solubility of gliadin and glutenin in water is an important factor in the…
Sequential Extraction and Quantitative Recovery of Gliadins, Glutenins, and Other Proteins from Small Samples of Wheat Flour
Sequential Extraction and Quantitative Recovery of Gliadins, Glutenins, and Other Proteins from Small Samples of Wheat Flour (1) The major flour protein types are the…
Involvement of gliadin, a component of wheat gluten, in increased intestinal permeability leading to non-steroidal anti-inflammatory drug-induced small-intestinal damage
Involvement of gliadin, a component of wheat gluten, in increased intestinal permeability leading to non-steroidal anti-inflammatory drug-induced small-intestinal damage (1) 25% of patients with rheumatoid…
Mouse anti-benzylpenicilloyl IgE monoclonal antibody: preparation, characterization and cross-reactivity
Mouse anti-benzylpenicilloyl IgE monoclonal antibody: preparation, characterization and cross-reactivity (1) Clinical therapy using fl-lactam antibiotics sometimes leads to allergy of the immediate type such as…
Immunoglobulin-E-binding epitopes of wheat allergens in patients with food allergy to wheat and in mice experimentally sensitized to wheat proteins
Immunoglobulin-E-binding epitopes of wheat allergens in patients with food allergy to wheat and in mice experimentally sensitized to wheat proteins (1) Differences in the recognition…
House dust mite allergen Der f 2 induces interleukin-13 expression by activating the PI3K/Akt pathway
House dust mite allergen Der f 2 induces interleukin-13 expression by activating the PI3K/Akt pathway (1) Asthma is an inflammatory disease of the airways in…
Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling
Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling (1) Myeloid differentiation-2 (MD2), is an LPS-binding member of the Toll-like receptor 4 (TLR4)…
A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Infammation with Airway
A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Infammation with Airway Remodeling (1) Each mouse models of allergic airways inflammation varies with…
Induction of Oral Tolerance by Pepsin-Digested Gliadin Retaining T Cell Reactivity in a Mouse
Induction of Oral Tolerance by Pepsin-Digested Gliadin Retaining T Cell Reactivity in a Mouse Model of Wheat Allergy (1) oral administration of intact allergens can…
Probiotics Supplements Reduce ER Stress and Gut Inflammation Associated with Gliadin Intake in a Mouse Model of Gluten Sensitivity
Probiotics Supplements Reduce ER Stress and Gut Inflammation Associated with Gliadin Intake in a Mouse Model of Gluten Sensitivity (1) In Celiac Disease (CD) patients,…
Oral sensitization to whey proteins induces age- and sex-dependent behavioral abnormality and neuroinflammatory responses in a mouse model of food allergy: a potential role of mast cells
Oral sensitization to whey proteins induces age- and sex-dependent behavioral abnormality and neuroinflammatory responses in a mouse model of food allergy: a potential role of…