An adapted passive model of anti-MPO dependent crescentic glomerulonephritis reveals matrix dysregulation and is amenable to modulation by CXCR4 inhibition (1) Anti-neutrophil cytoplasmic antibody (ANCA)…
Science
Modulation of the Th1/Th2 Bias by Lipopeptide and Saponin Adjuvants in Orally Immunized Mice
070922 Modulation of the Th1/Th2 Bias by Lipopeptide and Saponin Adjuvants in Orally Immunized Mice (1) On day 40 after three cluster applications, an increase…
CTLA-4 Signaling Regulates the Intensity of Hypersensitivity Responses to Food Antigens, but is Not Decisive in the Induction of Sensitization
CTLA-4 Signaling Regulates the Intensity of Hypersensitivity Responses to Food Antigens, but is Not Decisive in the Induction of Sensitization (1) Antagonizing CTLA-4 signaling enhances…
Mapping of myeloperoxidase epitopes recognized by MPO-ANCA using human-mouse MPO chimers
Mapping of myeloperoxidase epitopes recognized by MPO-ANCA using human-mouse MPO chimers (1) Antineutrophil cytoplasmic autoantibodies (ANCA) directed against myeloperoxidase (MPO) or proteinase 3 are associated…
Mechanisms of Wheat Allergenicity in Mice: Comparison of Adjuvant-Free vs.Alum-Adjuvant Models
Mechanisms of Wheat Allergenicity in Mice: Comparison of Adjuvant-Free vs.Alum-Adjuvant Models (1) The genetic modification of wheat may also potentially alter its allergenicity. Despite widespread…
ICOS ligand expression is essential for allergic airway hyperresponsiveness
ICOS ligand expression is essential for allergic airway hyperresponsiveness (1) In order to be activated, T cells need to receive three signals: TCR engagement, co-stimulatory…
Immune Checkpoint Inhibitor Therapy for Bone Metastases: Specific Microenvironment and Current Situation
Immune Checkpoint Inhibitor Therapy for Bone Metastases: Specific Microenvironment and Current Situation (1) The immune checkpoints, such as programmed cell death protein 1 (PD-1), programmed…
Development of a monoclonal antibody-based sandwich ELISA for the detection of ovalbumin in foods
Development of a monoclonal antibody-based sandwich ELISA for the detection of ovalbumin in foods. (1) The major allergens in egg white include ovalbumin (OVA), ovomucoid,…
Differences in allergenic potential of food extracts following oral exposure in mice reflect differences in digestibility: potential approaches to safety assessment
Differences in allergenic potential of food extracts following oral exposure in mice reflect differences in digestibility: potential approaches to safety assessment (1) Food allergies are…
BALB/c mice can be used to evaluate allergenicity of different food protein extracts
BALB/c mice can be used to evaluate allergenicity of different food protein extracts(1) The United States Food and Drug Administration (USFDA) reports the eight most…
A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M)
A novel marker for assessment of liver matrix remodeling: An enzyme-linked immunosorbent assay (ELISA) detecting a MMP generated type I collagen neo-epitope (C1M) (1) Liver…
A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat
A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat (1) Exposure to gluten proteins leads to damage of the…
A Improved extraction of prolamins for gluten detection in processed foods
A Improved extraction of prolamins for gluten detection in processed foods (1) Gluten intolerance is one of the most common food intolerances in the Western…
Increased Targeting of Donor Switch Region and IgE in S g1-Deficient B Cells
Increased Targeting of Donor Switch Region and IgE in S g1-Deficient B Cells.(1) In mice, S regions are organized in the following order: 59-Sm-Sg3-Sg1-Sg2b-Sg2a-Sε-Sa-39 and…
S region transcription per se promotes basal IgE class switch recombination but additional factors regulate the efficiency of the process
S region transcription per se promotes basal IgE class switch recombination but additional factors regulate the efficiency of the process. (1) During an immune response,…
The murine IgGI/IgE class switch program
The murine IgG1/IgE class switch program (1) In murine B cells immunoglobulin class switching to IgGl and IgE is controlled by the same cytokine, interleukin-4…
Frequency of Immunoglobulin E Class Switching Is Autonomously Determined and Independent of Prior Switching to Other Classes
Frequency of Immunoglobulin E Class Switching Is Autonomously Determined and Independent of Prior Switching to Other Classes (1) IgE switches recombina- tions, and the fact…
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids
Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids (1) The first of two PCRs creates a linear insert with plasmid…
An efficient system for high-level expression and easy purification of authentic recombinant protein
An efficient system for high-level expression and easy purification of authentic recombinant proteins (1) A fusion partner that has been used for some years is…
Cloning short DNA into plasmids by one-step PCR
Cloning short DNA into plasmids by one-step PCR(1) Several PCR-based cloning protocols have been proposed to skip the use of DNA ligases and restriction endonucleases….