Recombinant protein expression in Escherichia coli: advances and challenges (1) In glucose-salts media and given the optimal environmental conditions, E.coli’s doubling time is about 20…
Science
Strategies for the Production of Recombinant Protein in Escherichia coli
Strategies for the Production of Recombinant Protein in Escherichia coli (1) In E. coli, we face two main problems; (1) is difficult or little expression…
New tools for recombinant protein production in Escherichia coli: A 5-year update
New tools for recombinant protein production in Escherichia coli: A 5-year update (1) One derivative of B-lines, BL21(DE3) has become the preferred host for recombinant…
Role of Polymeric Immunoglobulin Receptor in IgA and IgM Transcytosis
Role of Polymeric Immunoglobulin Receptor in IgA and IgM Transcytosis (1) Only IgA and IgM can polymerize. The formation of IgA dimers and IgM pentamers…
BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes
BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes (1) B-cells perform these functions by producing antibodies, proteins that are either secreted or expressed on…
Purification and Characterization of Small Molecular Weight Myeloperoxidase from Human Promyelocytic Leukemia HL-60 Cells
Purification and Characterization of Small Molecular Weight Myeloperoxidase from Human Promyelocytic Leukemia HL-60 Cells (1) The human leukemia line HL-60 cells are promyelocytic cells and…
Conformational and thermal stability of mature dimeric human myeloperoxidase and a recombinant monomeric form from CHO cells
Conformational and thermal stability of mature dimeric human myeloperoxidase and a recombinant monomeric form from CHO cells (1) Myeloperoxidase is an enzyme that prefers small…
Evaluation of the efficiency of hydrolyzed whey formula to prevent cow’s milk allergy in the BALB/c mouse model
Evaluation of the efficiency of hydrolyzed whey formula to prevent cow’s milk allergy in the BALB/c mouse model (1) When Treg cells are not functional…
The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions
The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions (1) The murine myeloblastic cell line 32DCl3 is an interleukin-3 (IL-3)-dependent…
Bone morphogenetic proteins in breast cancer: dual role in tumourigenesis
Bone morphogenetic proteins in breast cancer: dual role in tumourigenesis? (1) Bone morphogenetic proteins (BMPs) are extracellular signaling molecules that constitute the largest branch of…
Mast cell heterogeneity underlies different manifestations of food allergy in mice
Mast cell heterogeneity underlies different manifestations of food allergy in mice (1) During the allergic response, food allergen cross-links IgE bound to MCs via FcεRI,…
Mast cells are required for experimental oral allergen–induced diarrhea
Mast cells are required for experimental oral allergen–induced diarrhea (1) Gastrointestinal allergic diseases are characterized by a wide array of manifestations, including elevated levels of…
Identification of a common Ara h 3 epitope recognized by both the capture and the detection monoclonal antibodies in an ELISA detection kit
Identification of a common Ara h 3 epitope recognized by both the capture and the detection monoclonal antibodies in an ELISA detection kit (1) Peanut…
Crystal structure of Ara h 3, a major allergen in peanut
Crystal structure of Ara h 3, a major allergen in peanut (1) Ara h 2 and Ara h 3 have been reported to have activities…
Murine model of food allergy after epicutaneous sensitization: Role of mucosal mast cell protease-1
Murine model of food allergy after epicutaneous sensitization: Role of mucosal mast cell protease-1 (1) Two main types of mast cells have been described; tryptase-releasing…
The Role of Neutrophils in the Induction of Glomerulonephritis by Anti-Myeloperoxidase Antibodies
The Role of Neutrophils in the Induction of Glomerulonephritis by Anti-Myeloperoxidase Antibodies (1) Anti-neutrophil cytoplasm autoantibodies (ANCAs) crescentic glomerulonephritis (NCGN) is the most common form…
Digestion and Transport across the Intestinal Epithelium Affects the Allergenicity of Ara h 1 and 3 but Not of Ara h 2 and 6
Digestion and Transport across the Intestinal Epithelium Affects the Allergenicity of Ara h 1 and 3 but Not of Ara h 2 and 6 (1)…
Antineutrophil cytoplasmic autoantibodies specific for myeloperoxidase cause glomerulonephritis and vasculitis in mice
Antineutrophil cytoplasmic autoantibodies specific for myeloperoxidase cause glomerulonephritis and vasculitis in mice (1) ANCAs are specific for antigens in the primary granules of neutrophils and…
Murine models for evaluating the allergenicity of novel proteins and foods
Murine models for evaluating the allergenicity of novel proteins and foods(1) The guidance from the Food and Agriculture Organization (FAO) and the World Health Organization…
Epitope specificity of myeloperoxidase antibodies: identification of candidate human immunodominant epitopes
Epitope specificity of myeloperoxidase antibodies: identification of candidate human immunodominant epitopes (1) Two major types of anti-neutrophil cytoplasmic antibodies: cytoplasmic ANCA (c-ANCA) and perinuclear ANCA…