Genetically Determined Severity of Anti-Myeloperoxidase Glomerulonephritis (1) Necrotizing crescentic glomerulonephritis (NCGN) is induced in mice by injecting anti-MPO IgG, and is mediated by neutrophils, enhanced…

Purification and molecular characterization of a truncated-type Ara h1, a major peanut allergen: oligomer structure, antigenicity, and glycoform (1) Ara h 1 consists of three…

Evaluation of an in vitro method for the measurement of specific IgE antibody responses: the rat basophilic leukemia (RBL) cell assay. (1) As IgE antibody…

A Single Monoclonal Antibody against the Peanut Allergen Ara h 2 Protects against Systemic and Local Peanut Allergy (1) For the induction of anaphylaxis, sensitized…

The Effect of the Food Matrix on In Vivo Immune Responses to Purified Peanut Allergens (1) Major food allergens, in general, are glycoproteins ranging from…

RBL-2H3 cells are an imprecise model for mast cell mediator release (1) Mast cells degranulate in response to peptidoglycan challenge through a TLR2-dependent pathway and…

Epicutaneous exposure to peanut protein prevents oral tolerance and enhances allergic sensitization(1) The key feature of the disease is a T-helper type 2 (Th2)-predominant allergen-…

Three-dimensional Models of Four Mouse Mast Cell Chymases (1) Seven different 26-32-kDa mast cell serineproteases,designated mouse mast cell protease(mMCP) 1 to mMCP-7, have been identified…

An efficient system for high-level expression and easy purification of authentic recombinant proteins (1) A fusion partner that has been used for some years is…

Selecting an Appropriate Method for Expressing a Recombinant Protein (1) E. coli is normally inefficient in promoting the correct formation of disulfide bonds when recombinant…

Recombinant protein production in bacterial hosts (1) The bacterial recombinant protein production process is outlined. Some commonly used promoter systems are described The pET system,…

Recombinant protein expression in Escherichia coli: advances and challenges (1) In glucose-salts media and given the optimal environmental conditions, E.coli’s doubling time is about 20…