In animal studies, Bauer et al. reported that prenatal and postnatal exposure to BPA enhanced allergic lung inflammation in female but not male offspring of…

IgM has been shown to direct systemic antibody response through the use of complement activation, especially in response to larger antagonists such as viruses or…

Chemical methods for transfection include the use of cationic liposomes (lipoplex), polymers (polyplex), combinations of the two (lipopolyplex), calcium phosphate, and DEAE dextran. In addition…

Neuroinflammatory responses can be mimicked in the CNS of rats treated with a bacterial component, that is, lipopolysaccharide (LPS) or the pro-inflammatory cytokine interleukin-1 (IL-1)….

The quality of standards for assays is crucial for obtaining consistent results throughout a long-term study and between studies. Using different batches of these reagents…

Type III collagen is vital for the formation of normal type I collagen fibrils in the cardiovascular system, intestines, and skin. In the extracellular matrix…

IL-10 was secreted by T-helper Th2 cell clones and inhibited cytokine pro- duction by Th1 cells. IL-10 was also found to be secreted by CD4…

The collagen solution at neutral pH was dialyzed against 0.05 M Tris-HCl buffer, pH 7.5, containing 1 M NaCl. The resulting solution was centrifuged, then…

Introduction A ELISA kit detects only native type II collagen and not denatured collagen. The sample preparation approach involves heat denaturation of collagen and the…

Introduction The Enzyme-Linked Immunosorbent Assay (ELISA) is a popular method used to detect and quantify soluble analytes such as proteins, hormones, and antibodies (1, 2)….

Use of Modified BL21(DE3) Escherichia coli Cells for High-Level Expression of Recombinant Peanut Allergens Affected by Poor Codon Usage (1) The cDNAs were subcloned into…

Characterization of Causative Allergens for Wheat-Dependent Exercise-Induced Anaphylaxis Sensitized with Hydrolyzed Wheat Proteins in Facial Soap (1) ω5-Gliadin and HMW-glutenin were identified as major allergens…

Nafamostat has anti-asthmatic effects associated with suppressed pro-inflammatory gene expression, eosinophil infiltration and airway hyperreactivity (1) Nafamostat is currently employed clinically for treating pancreatitis. However,…

Allergens from house dust and storage mites (1) in very humid housing conditions, storage mites of the species Lepidoglyphus destructor, Tyrophagus putrescentiae, and Glycypha-gusdomesticus are…

Intravital analysis of vascular permeability in mice using two-photon microscopy (1) A number of groups have used an in-vitro assay system that measures the flux…

Induction of Anti-DNA IgG and IgE Antibodies in BALB/c Mice (1) Varying amounts of reaginic IgE antibodies specific for native DNA, denatured DNA (dnDNA), RNA…

Intestinal permeation enhancers enable oral delivery of macromolecules up to 70 kDa in size (1) The small intestine is highly absorptive to small molecules and…

Acid Hydrolysis of Gluten Enhances the Skin Sensitising Potential and Drives Diversification of IgE Reactivity to Unmodified Gluten Proteins (1) It is well-accepted that hydrolysis…

An Adjuvant-Free Mouse Model Using Skin Sensitization Without Tape-Stripping Followed by Oral Elicitation of Anaphylaxis: A Novel Pre-Clinical Tool for Testing Intrinsic Wheat Allergenicity (1)…

Allergenicity of Deamidated and/or Peptide-Bond-Hydrolyzed Wheat Gliadin by Transdermal Administration (1) Regarding patients with wheat-dependent, exercise-induced anaphylaxis (WDEIA), certain tandem sequencing sites with glutamine residues…