In animal studies, Bauer et al. reported that prenatal and postnatal exposure to BPA enhanced allergic lung inflammation in female but not male offspring of…
Month: July 2024
Novel peptoid-based adsorbents for purifying IgM and IgG from polyclonal and recombinant sources
IgM has been shown to direct systemic antibody response through the use of complement activation, especially in response to larger antagonists such as viruses or…
Optimization of Gene Transfection in Murine Myeloma Cell Lines using Different Transfection Reagents
Chemical methods for transfection include the use of cationic liposomes (lipoplex), polymers (polyplex), combinations of the two (lipopolyplex), calcium phosphate, and DEAE dextran. In addition…
Anti-inflammatory effect by lentiviral-mediated overexpression of IL-10 or IL-1 receptor antagonist in rat glial cells and macrophages
Neuroinflammatory responses can be mimicked in the CNS of rats treated with a bacterial component, that is, lipopolysaccharide (LPS) or the pro-inflammatory cytokine interleukin-1 (IL-1)….
Monomeric and polymeric collagen in immunological assays
The quality of standards for assays is crucial for obtaining consistent results throughout a long-term study and between studies. Using different batches of these reagents…
How to prepare and analyze type I and type III collagen using a SDS-PAGE.
Type III collagen is vital for the formation of normal type I collagen fibrils in the cardiovascular system, intestines, and skin. In the extracellular matrix…
In Situ Identification of Both IL-4 and IL-10 Cytokine–Receptor Interactions during Tissue Regeneration
IL-10 was secreted by T-helper Th2 cell clones and inhibited cytokine pro- duction by Th1 cells. IL-10 was also found to be secreted by CD4…
Separation of the a Chains of Type I and III Collagens by SDS-Polyacrylamide Gel Electrophoresis
The collagen solution at neutral pH was dialyzed against 0.05 M Tris-HCl buffer, pH 7.5, containing 1 M NaCl. The resulting solution was centrifuged, then…
Native Collagen Detection by Type II collagen ELISA
Introduction A ELISA kit detects only native type II collagen and not denatured collagen. The sample preparation approach involves heat denaturation of collagen and the…
Understanding the Hook Effect in a One-Step Sandwich ELISA
Introduction The Enzyme-Linked Immunosorbent Assay (ELISA) is a popular method used to detect and quantify soluble analytes such as proteins, hormones, and antibodies (1, 2)….