Increased Targeting of Donor Switch Region and IgE in S g1-Deficient B Cells.(1) In mice, S regions are organized in the following order: 59-Sm-Sg3-Sg1-Sg2b-Sg2a-Sε-Sa-39 and…

S region transcription per se promotes basal IgE class switch recombination but additional factors regulate the efficiency of the process. (1) During an immune response,…

The murine IgG1/IgE class switch program (1) In murine B cells immunoglobulin class switching to IgGl and IgE is controlled by the same cytokine, interleukin-4…

Frequency of Immunoglobulin E Class Switching Is Autonomously Determined and Independent of Prior Switching to Other Classes (1) IgE switches recombina- tions, and the fact…

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids (1) The first of two PCRs creates a linear insert with plasmid…

An efficient system for high-level expression and easy purification of authentic recombinant proteins (1) A fusion partner that has been used for some years is…

Cloning short DNA into plasmids by one-step PCR(1) Several PCR-based cloning protocols have been proposed to skip the use of DNA ligases and restriction endonucleases….

Strategies for the Production of Recombinant Protein in Escherichia coli (1) A protein’s intrinsic ability to fold, its solubility, preferential codon use, its toxicity, and…

Cloning technologies for protein expression and purification(1) Protein overexpression requires three components: a gene, a vector that contains the gene, and an expression host that…

BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity (1) IgAs bind to microbial components…

Airway epithelial cell necroptosis contributes to asthma exacerbation in a mouse model of house dust mite-induced allergic inflammation (1) Recent research has highlighted the importance…

IgA mesangial deposits in C3H/HeJ mice after oral immunization with ferritin or bovine serum albumin(1) The IgA deposited in the kidney are polymeric and presumably…

EXPERIMENTAL IgA NEPHROPATHY INDUCED BY ORAL IMMUNIZATION* (1) An expansion of specific IgA synthesizing cells in mesenteric lymph nodes,spleen,and secretory mucosae and a significant elevation…

Experimental Immunoglobulin A Nephropathy Induced by Gram-Negative Bacteria (1) On the basis of clinical and experimental observations, a number of etiologic candi­dates for IgA nephropathy…

Mice Refractory to Lipopolysaccharide Manifest High Immunoglobulin A Responses to Orally Administered Antigen (1) The interaction of antigen with gut-associated lymphoid tissues is a major…

Epitope characterization of ovalbumin in BALB/c mice using different entry routes (1) Ovalbumin is a 385 amino acid glycoprotein of approximately 45 kDa, which constitutes…

Salt-Induced Disaggregation/Solubilization of Gliadin and Glutenin Proteins in Water (1) The limited solubility of gliadin and glutenin in water is an important factor in the…

Sequential Extraction and Quantitative Recovery of Gliadins, Glutenins, and Other Proteins from Small Samples of Wheat Flour (1) The major flour protein types are the…

Involvement of gliadin, a component of wheat gluten, in increased intestinal permeability leading to non-steroidal anti-inflammatory drug-induced small-intestinal damage (1) 25% of patients with rheumatoid…

Mouse anti-benzylpenicilloyl IgE monoclonal antibody: preparation, characterization and cross-reactivity (1) Clinical therapy using fl-lactam antibiotics sometimes leads to allergy of the immediate type such as…