Oral allergy syndrome and anaphylactic reactions in BALB/c mice caused by soybean glycinin and b-conglycinin

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Oral allergy syndrome and anaphylactic reactions in BALB/c mice caused by soybean glycinin and beta-conglycinin (1)

Soybean glycinin and b-conglycinin represent up to one-third of the protein in soybean, and have been identified as binding IgE of subjects with soy allergy. Increased soybean-specific IgE and IgG antibodies were observed in BALB/c mice sensitized by gavage daily in the presence of an adjuvant.

Mice were sensitized by an intragastric (i.g.) administration of 1 mg of soybean glycinin or beta-conglycinin/1mL/mouse daily for 5 weeks.

The serum titres of soybean glycinin- or beta-conglyinin-specific IgE and IgG1 significantly increased (P < 0.01) in mice sensitized with an oral administration of soybean glycinin or beta-conglyinin by gavage.

The administra- tion of glycinin and beta-conglyinin resulted in a rapid increase of serum histamine in BALB/c mice, with maximal release within 28 days. Although they were somewhat decreased after 28 days in the glycinin or the beta-conglyinin-gavaged group. The percentile of intact mast cells of the small intestine in mice sensitized with glycinin or beta-conglyinin significantly decreased during 28 days.

The level of blood pressure in mice sensitized with glycinin or beta-conglyinin reached the minimum at 3 hours.

The serum histamine level increased after protein-antigen challenge using gavage daily in BALB/c mice.

1. X. Liu, J. Feng, Z. R. Xu, Y. Z. Wang, J. X. Liu, Oral allergy syndrome and anaphylactic reactions in BALB/c mice caused by soybean glycinin and beta-conglycinin. Clin. Exp. Allergy. 38, 350–356 (2008).

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