In vivo and in vitro IgE isotype switching in human B lymphocytes: Evidence for a predominantly direct IgM to IgE class switch program (1) Isotype…
Month: June 2021
Wheat Varietal Identification by Rapid Ion-Exchange Chromatography of Gliadins
Wheat Varietal Identification by Rapid Ion-Exchange Chromatography of Gliadins (1) A rapid ion-exchange chromatography system for proteins has also become available. This system, known as…
Large-scale preparation of gliadin proteins
Large-scale preparation of gliadin proteins (1) The column (22 x 10 cm), packed with CM 52 cellulose (1.7 kg), was equilibrated for 24 h with…
Identification and Mutational Analysis of the Immunodominant IgE Binding Epitopes of the Major Peanut AllergenAra h 2
Identification and Mutational Analysis of the Immunodominant IgE Binding Epitopes of the Major Peanut Allergen Ara h 2 (1) Two highly abundant glycoproteins found in…
Purification and immunoglobulin E-binding properties of peanut allergen Ara h 6: evidence for cross-reactivity with Ara h 2
Purification and immunoglobulin E-binding properties of peanut allergen Ara h 6: evidence for cross-reactivity with Ara h 2 (1) Peanut allergens Ara h 1, Ara…
Purification and characterisation of a panel of peanut allergens suitable for use in allergy diagnosis
Purification and characterisation of a panel of peanut allergens suitable for use in allergy diagnosis (1) Proteins were extracted by stirring 40 g of defatted…
Generation and Characterization of Monoclonal Antibodies Against the Intracellular Domain of Hemidesmosomal Type XVII Collagen
Generation and Characterization of Monoclonal Antibodies Against the Intracellular Domain of Hemidesmosomal Type XVII Collagen (1) Type XVII collagen, also termed bullous pemphigoid antigen 2…
Cleavage of BP180, a 180-kDa Bullous Pemphigoid Antigen, Yields a 120-kDa Collagenous Extracellular Polypeptide
Cleavage of BP180, a 180-kDa Bullous Pemphigoid Antigen, Yields a 120-kDa Collagenous Extracellular Polypeptide (1 There are at least three hemidesmosomal transmembrane polypeptides, i.e. integrin…
Experimental bullous pemphigoid generated in mice with an antigenic epitope of the human hemidesmosomal protein BP230
Experimental bullous pemphigoid generated in mice with an antigenic epitope of the human hemidesmosomal protein BP230 (1) Immunodominant and pathogenic epitopes of BP180 associated with…
Autoantibodies to BPAG1e trigger experimental bullous pemphigoid in mice
Autoantibodies to BPAG1e trigger experimental bullous pemphigoid in mice (1) Bullous pemphigoid (BP) is the most common subepidermal autoimmune blistering disease characterized by autoantibodies in…
Human IgG1 Monoclonal Antibody against Human Collagen 17 Noncollagenous 16A Domain Induces Blisters via Complement Activation in Experimental Bullous Pemphigoid Model
Human IgG1 Monoclonal Antibody against Human Collagen 17 Noncollagenous 16A Domain Induces Blisters via Complement Activation in Experimental Bullous Pemphigoid Model (1) Bullous pemphigoid (BP)…
Bullous Pemphigoid Autoantibodies Directly Induce Blister Formation without Complement Activation
Bullous Pemphigoid Autoantibodies Directly Induce Blister Formation without Complement Activation (1) Studies to map the epitopes on COL17 have revealed that the juxtamembranous extracellular noncollagenous…
Passive transfer of collagen XVII-specific antibodies induces sustained blistering disease in adult mice
Passive transfer of collagen XVII-specific antibodies induces sustained blistering disease in adult mice (1) Bullous pemphigoid (BP) is characterized by the presence of tissue-bound and…
Peanut protein concentrate: Production and functional properties as affected by processing
Peanut protein concentrate: Production and functional properties as affected by processing(1) Vegetable oil extraction from peanut yields partially defatted peanut flour (DPF). DPF is protein-rich,…
Structures of T Cell Immunoglobulin Mucin Receptors 1 and 2 Reveal Mechanisms for Regulation of Immune Responses by the TIM Receptor Family
Structures of T Cell Immunoglobulin Mucin Receptors 1 and 2 Reveal Mechanisms for Regulation of Immune Responses by the TIM Receptor Family (1) mTIM-1 is…
Processing Can Alter the Properties of Peanut Extract Preparations
Processing Can Alter the Properties of Peanut Extract Preparations (1) Several different enzyme-linked immunosorbent assay (ELISA) methods are being used to detect either the common…
Peanut protein extraction conditions strongly influence yield of allergens Ara h 1 and 2 and sensitivity of immunoassays
Peanut protein extraction conditions strongly influence yield of allergens Ara h 1 and 2 and sensitivity of immunoassays(1) Extraction is the basis for all subsequent…
Epicutaneous immunotherapy on intact skin using a new delivery system in a murine model of allergy
Epicutaneous immunotherapy on intact skin using a new delivery system in a murine model of allergy (1) Viaskin is a new epicutaneous delivery system (EDS)…
A tractable covalent linker strategy for the production of immunogenic antigen-TLR7/8L bioconjugates
A tractable alent linker strategy for the production of immunogenic antigen-TLR7/8L bioconjugates (1) Direct covalent attachment of Toll-like receptor (TLR) agonists to antigenic proteins has…
Blocking monoclonal antibodies of TIM proteins as orchestrators of anti-tumor immune response
Blocking monoclonal antibodies of TIM proteins as orchestrators of anti-tumor immune response (1) T cell immunoglobulin and mucin domain (TIM) proteins are type-I cell-surface molecules…