Culturing hybridoma is sometimes challenging. The growth rate and antibody production vary in individual clones. We generally choose hybridoma who has fast growth and high antibody production, however, for a long period of the cell culture, hybridoma suddenly stops growing and producing antibodies. In this situation, supplements will help for maintaining hybridoma. The Bricone containing IL-6 can be used for recovering hybridoma as well as cloning and post-cell fusion(1, 2).

1. R. Weedle, K. Carroll, K. Kavanagh, R. O’Kennedy, M. Clynes, in Animal Cell Technology, R. E. Spier, J. B. Griffiths, W. Berthold, Eds. (Butterworth-Heinemann, 1994), pp. 96–98.

2. S. Pandey, Hybridoma technology for production of monoclonal antibodies. Hybridoma (2010) (available at http://www.academia.edu/download/50053256/hybridoma_tech_1.pdf).