Since C57BL/6 mice are a low susceptible strain to collagen induced arthritis (CIA), the arthritis model requires high concentration of M.tuberculosis in Complete Freund’s Adjuvant (CFA) for immunization with a booster injection to induce CIA (1). The chick type II collagen and 5 mg/ml CFA must be used to make emulsion for the initial and booster immunization. The report apparently showed that the high concentration of M.tuberculosis must be used to induce CIA (2). The reference can be used for applying our study protocol to the animal committee to obtain their approval.

1. I. K. Campbell, J. A. Hamilton, I. P. Wicks, Collagen-induced arthritis in C57BL/6 (H-2b) mice: new insights into an important disease model of rheumatoid arthritis. Eur. J. Immunol. 30, 1568–1575 (2000).

2. H. Kai, K. Shibuya, Y. Wang, H. Kameta, T. Kameyama, S. Tahara-Hanaoka, A. Miyamoto, S.-I. Honda, I. Matsumoto, A. Koyama, T. Sumida, A. Shibuya, Critical role of M. tuberculosis for dendritic cell maturation to induce collagen-induced arthritis in H-2b background of C57BL/6 mice. Immunology. 118, 233–239 (2006).